In vivo Hepatoprotective Potential of Nigella sativa Extract Against Rifampicin Induced Sub-chronic Hepatotoxicity and Altered Redox Status

In vivo Hepatoprotective Potential of Nigella sativa Extract Against Rifampicin Induced Sub-chronic Hepatotoxicity and Altered Redox Status

Journal of Biologically Active Products from Nature

Volume 2, Issue 3, 2012

DOI:

10.1080/22311866.2012.10719124

Devendra Singh kushwah^a, Mohd Tariq Salman^a*, Hemant Kumar Singh^a, Ali Ahmad^a & V.K. verma^a 

pages 167-177

Despite the hepatotoxic effects of Rifampicin (RIF), its use is inevitable in the management of tuberculosis. No satisfactory treatment is available for prevention of this adverse effect. This study was undertaken to evaluate the protective effect of Nigella sativa extract (NSE) against Rifampicin induced sub-chronic hepatotoxicity. Male wistar rats were divided in 4 groups of 8 each and received vehicle, RIF (100 mg/ kg), RIF (100 mg/kg)+NSE (250 mg/kg) or RIF (100 mg/kg)+NSE (500 mg/kg) orally respectively for 28 days. Blood was withdrawn at day 14 and 28 for estimation of circulatory liver markers and anti-oxidant levels. At the end of the experiment histopathological study and estimation of SOD, CAT, GSH and LPO in liver samples was done. Pre-treatment of NSE produced significant hepatoprotection by decreasing the level of serum Alkaline phosphatase (ALP) (p< 0.01), Serum glutamic pyruvic transaminase (SGPT) (p< 0.001), Serum glutamic oxaloacetic transaminase (SGOT) (p<0 .001="" action="" advocate="" against="" along="" and="" another="" architecture="" bilirubin="" bridging="" by="" cat="" changes="" chronic="" degeneration="" degenerative="" drug="" extending="" fatty="" from="" further="" group="" groups="" gsh="" hepatocytes="" hepatoprotective="" hepatotoxicity.="" hepatoxicity.="" histopathology="" in="" increasing="" indicate="" induced="" infiltration="" is="" its="" kg="" level="" levels="" liver="" mda="" mg="" microvesicular="" mononuclear="" near="" necrosis="" needed="" normal="" nse="" of="" one="" p="" persisting="" portal="" possesses="" pre-treatment="" prevention="" regaining="" research="" results="" rif="" serum="" showed="" sod="" span="" steatosis.="" sub="" that="" the="" to="" tract.="" tract="" triditis="" use="" with="">

Black cumin seeds possess analgesic and anti-inflammatory activity comparable to indomethacin.

IN VIVO EVALUATION OF ANTI-INFLAMMATORY AND ANALGESIC ACTIVITIES OF NIGELLA SATIVA SEED DURING GERMINATION

MOHAMMAD HAYATUL ISLAM, IFFAT ZAREEN AHMAD AND MOHAMMAD TARIQ SALMAN

Objective: The medicinal values of Nigella sativa have been mentioned in ancient literature as useful in disorders of inflammation. The present study investigates the anti-inflammatory and analgesic activities of methanol extract of Nigella sativa seed during different phases of its germination on Wistar rats. Methods: Seeds of N. sativa were grown in vitro in glass petri plates using multiple folds of damp filter paper. Complete plantlet with two leaves was obtained in 11 days. The acute anti-inflammatory activity of N. sativa extracts during different phases of germination was measured plethysmographically using kaolin as inflammatory agent, analgesic activity was measured by hot plate method, keeping indomethacin (10mg/kg b.w) as reference standard in both tests.
Results: All tested extracts of N. sativa (1gm/kg b.w) during different phases of germination showed significant reduction in paw oedema in comparison to control (P<0 .001="" 5th="" all="" among="" analgesic="" and="" anti-inflammatory="" day="" during="" effect="" extract="" extracts.="" germination="" hot="" in="" increased="" of="" on="" p="" plate="" reaction="" showed="" significant="" test.="" test="" tested="" the="" time="">
Conclusion: It may be concluded that extracts of N. sativa possess enhanced anti-inflammatory and analgesic activities during germination as compared to seed extract. High metabolic activity and higher contents of secondary metabolites expressed during germination might be responsible for this activity.

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Black Cumin seed (Nigella sativa, Kalonji) protects against Paracetamol induced hepatotoxicity

Protective Effects of Ethanolic Extract of Nigella sativa Seed in Paracetamol Induced Acute Hepatotoxicity In vivo

D.S. Kushwah, M.T. Salman, P. Singh, V.K. Verma and A. Ahmad

Paracetamol overdose causes serious liver necrosis. Hepatoprotective activity of ethanolic extract of Nigella sativa in Paracetamol induced acute hepatotoxicity was investigated in rats. Fasted male Wistar rats were orally treated with Nigella sativa extract in graded doses for 5 days followed by Nigella sativa extract and paracetamol 3 g kg-1 on 6 and 7th day. Circulatory liver markers and reduced glutathione (GSH) levels were estimated and histopathological study of liver performed. Paracetamol caused a significant increase in serum alkaline phosphatase, glutamic oxaloacetic transaminase, glutamic pyruvic transaminase and total Bilirubin and a significant decrease in GSH compared to control. Nigella sativa pretreatment significantly prevented the increase in liver enzymes and total bilirubin and decrease in GSH level as compared to paracetamol group. Liver histopathology showed marked reduction in sinusoidal dilatation, midzonal necrosis, portal triaditis and occasional apoptosis in Nigella sativa extract treated groups as compared to group receiving only paracetamol. Nigella sativa extract possesses hepatoprotective action against paracetamol induced acute hepatoxicity. Further research is needed to advocate its prophylactic use for drug induced hepatotoxicity

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Treatment of progressive, metastatic medullary thyroid cancer.

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Antibacterial activity of Nigella sativa seed in various germination phases on clinical bacterial strains isolated from human patients

See full text of paper on antimicrobial activity of N. sativa in various germination stages against clinical strains. Nigella sativa is an important spice and flavoring agent which is widely used in various European and Asian cuisines. It harbors an array of medicinal properties as shown by various researches. Germination is a phenomenon during which rapid changes in metabolic activities and the interconversions of metabolites take place. The objective of present study was to evaluate the antibacterial activity of N. sativa seed that are on various germination phases against clinical bacterial strains isolated from pus, urine, ascitic fluid and cerebrospinal fluid of various patients. The minimum inhibitory concentration (MIC) values were determined by using a modified macro-broth dilution technique. The agar well diffusion method was used to test the antimicrobial effects of N. sativa extracts. Some broad spectrum antibiotics were used as positive control. The phytochemical constituents of N. sativa seed were also studied in germination phases. The distilled methanolic extracts of N. sativa showed significant antimicrobial activity against tested clinical strains of Gram-positive Staphylococcus aureus and Gram-negative Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa and Proteus mirabilis bacteria. Results showed day-dependent and dose-dependent activity and a significant antimicrobial effect was observed as germination proceeded.

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Knowledge, Attitudes and Practices of Bioethics among Doctors in a Tertiary Care Government Teaching Hospital in India
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http://dx.doi.org/10.4172/2155-9627.1000118

Drugs Update

New drugs approved for marketing in India during November-December 2011

Source: http://cdsco.nic.in

Diazinon Flea Drops Spot on Dogs 30.0% w/w	For dogs infected with fleas.
Cabazitaxel Injection 60mg / 1.5ml	In combination with prednisone for treatment of patients with hormone-refractory metastatic prostrate cancer previously treated with a docetaxel-containing treatment regimen.
Trifluridine Eye Drops 1% w/v	For the treatment of primary keratoconjunctivitis and recurrent epithelial keratitis due to herpes simplex viruses type 1 and 2.
Fosaprepitant (as Dimeglumine) for Injection 150mg.	1. Prevention of acute and delayed nausea and vomiting associated with highly emetogenic cisplatin based cancer chemotherapy in adults. 2. Prevention of nausea and vomiting associated with moderately emetogenic cancer chemotherapy in adults.
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Crizotinib  hard gelatin Capsules 200mg/250mg	For the treatment of patients with locally advanced or metastatic non-small cell lung cancer (NSCLC) that is anaplastic lymphoma kinase (ALK) – positive as detected by an FDA-approved test.

Drugs  banned in India in 2011

Source: http://cdsco.nic.in

1.      Gatifloxacin

2.      Tegaserod

3.      Nimesulide for children below 12 years.

4.      Cisapride

5.      Phenyl propanolamine

6.      Human placental extract

7.      Sibutramine

8.      R-Sibutramine

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Source: http://drugs.com

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Inducer of cell death indicated for the topical treatment of actinic keratosis.

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Kinase inhibitor indicated for the treatment of advanced renal cell carcinoma

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7.      Vismodegib Capsules

Hedgehog pathway inhibitor for the treatment of patients with advanced basal cell carcinoma (BCC).

8.      Ivacaftor Tablets

Cystic fibrosis transmembrane conductance regulator (CFTR) potentiator indicated for the treatment of cystic fibrosis (CF) in patients age 6 years and older who have a G551D mutation in the CFTR gene.

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