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Showing posts with label CAM. Show all posts
Showing posts with label CAM. Show all posts

Wednesday, August 12, 2015

Neuroprotective effects of Nigella sativa extracts during germination on central nervous system


Background: Nigella sativa Linn. which has many acclaimed medicinal properties is an indigenous herbaceous plant and belongs to the Ranunculaceae family, which grows in countries bordering the Mediterranean Sea, Pakistan and India. Objective: This study was designed to investigate the effects of N. sativa seed extracts of different germination phases on the central nervous system (CNS) responses in experimental animals. 
Materials and Methods: Anxiolytic, locomotor activity of extracts (1 g/kg of body weight) was evaluated in both stressed and unstressed animal models and antiepileptic effect was evaluated by maximal electroshock seizure model keeping diazepam (20 mg/kg) as a positive control. Antidepressant effect was evaluated by forced swim test and tail suspension test keeping imipramine (15 mg/kg) as a positive control. 
Results: All tested extracts of N. sativa during different phases of germination (especially 5th day germination phase) showed significant (P < 0.001) anxiolytic effect in comparison to control. Diazepam reduced locomotor activity in control (unstressed) rats but did not show affect in stressed rats while N. sativa extracts from germination phases significantly (P < 0.001) reduced locomotor activity in unstressed as well as stressed animals. All the extracts of N. sativa from different germination phases exhibited significant (P < 0.001) reduction in various phases of epileptic seizure on comparison with the reference standard (diazepam). During antidepressant test, N. sativa extracts exhibited a slight reduction in the immobility of rats. 
Conclusion: During germination, especially in 5th day germination extract, N. sativa showed significant CNS depressant activity as compared to whole seeds that possibly may be due higher content of secondary metabolites produced during germination.

Friday, October 18, 2013

Black cumin seeds possess analgesic and anti-inflammatory activity comparable to indomethacin.

IN VIVO EVALUATION OF ANTI-INFLAMMATORY AND ANALGESIC ACTIVITIES OF NIGELLA SATIVA SEED DURING GERMINATION

MOHAMMAD HAYATUL ISLAM, IFFAT ZAREEN AHMAD AND MOHAMMAD TARIQ SALMAN

Objective: The medicinal values of Nigella sativa have been mentioned in ancient literature as useful in disorders of inflammation. The present study investigates the anti-inflammatory and analgesic activities of methanol extract of Nigella sativa seed during different phases of its germination on Wistar rats. Methods: Seeds of N. sativa were grown in vitro in glass petri plates using multiple folds of damp filter paper. Complete plantlet with two leaves was obtained in 11 days. The acute anti-inflammatory activity of N. sativa extracts during different phases of germination was measured plethysmographically using kaolin as inflammatory agent, analgesic activity was measured by hot plate method, keeping indomethacin (10mg/kg b.w) as reference standard in both tests.
Results: All tested extracts of N. sativa (1gm/kg b.w) during different phases of germination showed significant reduction in paw oedema in comparison to control (P<0 .001="" 5th="" all="" among="" analgesic="" and="" anti-inflammatory="" day="" during="" effect="" extract="" extracts.="" germination="" hot="" in="" increased="" of="" on="" p="" plate="" reaction="" showed="" significant="" test.="" test="" tested="" the="" time="">
Conclusion: It may be concluded that extracts of N. sativa possess enhanced anti-inflammatory and analgesic activities during germination as compared to seed extract. High metabolic activity and higher contents of secondary metabolites expressed during germination might be responsible for this activity.

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Thursday, April 9, 2009

Novel agents and approaches for breast cancer

Cytotoxic chemotherapy remains an important part of the treatment paradigm for breast cancer. Anthracyclines and taxanes are the most active agents; however, limitations with their use include a maximum lifetime dose and tumor resistance with anthracycline, hypersensitivity reactions and cumulative toxicity with taxanes.
  • Therefore, to meet these challenges, the development of new cytotoxics and novel taxane formulations is an important area of active research. Several recent advances have been made. Epothilones represent a novel group of cytotoxic agents, with proven activity in breast cancer.
  • Nanoparticle drug delivery systems have led to the development of ABI-007, which has demonstrated superior response rates than 3-weekly paclitaxel, with a lower risk of hypersensitivity reactions.
  • To circumvent the problem of taxane resistance, larotaxel, a semisynthetic taxoid, and vinflunine, a synthetic vinca alkaloid, have been developed with encouraging clinical results to date.
  • Eribulin, a synthetic derivative of halichondrin has recently entered Phase III trials based on encouraging activity in heavily pretreated patients.
  • A further novel approach is the conjugation of cytotoxic agents to targeted agents, such as with trastuzumab-MCC-DM1.

Tuesday, May 13, 2008

How Does Acupuncture Work?

Nerve-Reflex Theory
This theory was developed by Japanese doctors of physiology (Ishikawa and Fujita et al ) who were deeply involved in acupuncture research in 1950s. It holds that when an abnormal condition occurs in an internal organ, alterations take place in the skin and muscles related to that organ by means of the autonomic nervous system. The autonomic nervous system extending through the internal organs, skin, subcutaneous tissues and muscles, constantly transmit information about the physical condition to the spinal cord and the brain. These information impulses set up a reflex action that cause symptoms of internal organ's disorders to manifest themselves on the surface area as hyper or hyposensitivity, spasm, contraction, tightness or lumping of the muscles, discolouration, dryness or oiliness of the skin,etc. This phenomena is called "Viscera-Cutanous Refelx". At the same time, this intimate relation can exert the reverse effects too. That is, stimulation of the skin and muscles can cause dilation or contraction of the vessels to change the blood and lymph flow of the internal organs, activate the endocrine (hormone) and immune system,etc. This phase is called "Cutanous -Viscera Reflex". According to this theory, Acupuncture is defined as the most effective systematized stimulation therapy to utilize these reflexes for restoring the homeostasis of the body and acceralate the healing process.
The Gate Control Theory of Pain
In the west, especially in the USA, media has popularized the amazing effect of acupuncture for pain relief, therefore, it is understandable that most scientific research has been focussed on the mechanism of acupuncture for pain relief In 1965 Drs. Melzack and Wall won scientific acclaim by proposing a theory called "Gate Control Theory of Pain", which was eventually hailed to be the best model to explain the mechanism of acupuncture for pain relief. This theory was the first serious attempt to unify the many ideas that existed about the mechanism that perceives and transmits pain through the nervous system. It holds that there are some specific nerve fibres that transmit pain to the spinal cord, while the input of other nerve fibres inhibits the transmission of pain. Both of these groups of fibres meet at an area called the "substantia gelatinous" in the spinal cord. The substantia gelatinous controls the integration of pain and pain inhibitory stimuli. If the pain input is excessive, then it is transmitted up the spinal cord to the brain to be perceived as pain. This theory proposes a balance between stimulation of the pain fibres and inhibition of that stimulus, so that pain is perceived only if the pain input overrides the inhibition of pain. The problem with this theory is that it does not explain the full spectrum of the effects of acupuncture. Acupuncture excites the pain inhibitory nerve fibres for a short period of time, thereby blocking pain, but the effects of acupuncture can last much longer than that, quite often for some months after the acupuncture needle has been removed, and nothing in the Gate Control Theory really explains this prolonged effect. The mechanism of pain perception and transmission has not been clearly and completely defined, and in the light of the current state of knowledge about the basic mechanism of pain, it is a little unreasonable to expect an explanation of the effects of acupuncture on pain.
Endorphin
The worsening problem of drug addiction in the West has provided a great impetus for research into the mechanism of morphine action and addiction. (Morphine, heroin and opium are all related chemicals, collectively called opiates.) Morphine-like substances have recently been discovered in the central nervous system (the brain and the spinal cord). These substances are called endorphin, or naturally-occurring morphine,, and they have been found to be very effective in blocking pain. It is also verified that acupuncture stimulation can trigger the release of endorphin into the central nervous system, and the effects of acupuncture anaesthesia can be reversed by the use of anti-morphine drugs. However, recent research shows that not all types of acupuncture anaesthesia can be blocked by anti-morphine. e.g. If a patient has a painful arthritic knee, and acupuncture provides relief for the knee pain, then anti-morphine do not usually block the effects of this type of acupuncture therapy. The effects of acupuncture can be very swift (in terms of seconds) and it seems that the release of chemicals might possibly be too slow a process to have such a swift action. The endorphin theory again only deals with pain and makes little attempt to explain the use and effects of acupuncture in non-painful diseases. Implicit Order of "X-Signal System"
This is one of the latest theories proposed by Dr. Yoshio Manaka (1911-1989), an internationally renowned MD (surgeon & acupuncturist) who had made great contribution in both clinical applications and research of Japanese acupuncture for almost 40 years. This theory holds that acupuncture works not only on physiological systems of the body but also would work on bio-informational system or "X-Signal System" with a primary regulatory function; primarily hemostatic functions, regulating the overall energetic condition and the overall physiological condition. This information system is essentially not anatomical (though in microscopic structures it may have some physical expression), just as the various theories of the "Qi", meridian (energy channel), yin-yang, and the five phases are not clearly anatomical. This information system can be described as being hidden or enfolded or implicit order of primitive biological properties which might arose through various stages of evolution and were then masked by the development of the more efficient automation systems such as the nervous and endocrine systems. This process of enfoldment probably involves their being absorbed into the body of information stored, for example, in the genetic information of DNA. These potential information could be unfolded only with the correct internal and external stimulus, and in that process we can see manifestations of the clinical laws of acupuncture that were classically formulated as "Qi", meridian, ying-yang, five phase, and biorythmic theories. Treatment that takes advantage of, and activate changes in this system, can produce diverse effects because potentially the whole biological system can be affected. If the function of this information system stays in the ideal condition, then the body will function optimally. Since Dr.Manaka was a remarkable figure equipped with profound, diverse knowledge and experiences of both traditional oriental medicine and modern scientific medicine, this model is very convincing and useful to explain various phenomena surrounding acupuncture. However, we still do not have scientific means to prove this model. We might have to wait until much more sophisticated high-tech machines or instruments will prove this theory in the future, like Einstein's "General Theory of Relativity" was finally confirmed many years later after its publication.
Other Ideas
Some research workers, particularly in Russia, have suggested that fields of biological activity exist around all living objects, which is often described as "Kirlian phenomena". The concept of 'biofields' has little hard scientific evidence to support it, but there are people in many countries now who are suggesting that acupuncture may work through these fields. At present there are no good grounds for accepting or rejecting these theories.

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