In vivo Hepatoprotective Potential of Nigella sativa Extract Against Rifampicin Induced Sub-chronic Hepatotoxicity and Altered Redox Status

In vivo Hepatoprotective Potential of Nigella sativa Extract Against Rifampicin Induced Sub-chronic Hepatotoxicity and Altered Redox Status

Journal of Biologically Active Products from Nature

Volume 2, Issue 3, 2012

DOI:

10.1080/22311866.2012.10719124

Devendra Singh kushwah^a, Mohd Tariq Salman^a*, Hemant Kumar Singh^a, Ali Ahmad^a & V.K. verma^a 

pages 167-177

Despite the hepatotoxic effects of Rifampicin (RIF), its use is inevitable in the management of tuberculosis. No satisfactory treatment is available for prevention of this adverse effect. This study was undertaken to evaluate the protective effect of Nigella sativa extract (NSE) against Rifampicin induced sub-chronic hepatotoxicity. Male wistar rats were divided in 4 groups of 8 each and received vehicle, RIF (100 mg/ kg), RIF (100 mg/kg)+NSE (250 mg/kg) or RIF (100 mg/kg)+NSE (500 mg/kg) orally respectively for 28 days. Blood was withdrawn at day 14 and 28 for estimation of circulatory liver markers and anti-oxidant levels. At the end of the experiment histopathological study and estimation of SOD, CAT, GSH and LPO in liver samples was done. Pre-treatment of NSE produced significant hepatoprotection by decreasing the level of serum Alkaline phosphatase (ALP) (p< 0.01), Serum glutamic pyruvic transaminase (SGPT) (p< 0.001), Serum glutamic oxaloacetic transaminase (SGOT) (p<0 .001="" action="" advocate="" against="" along="" and="" another="" architecture="" bilirubin="" bridging="" by="" cat="" changes="" chronic="" degeneration="" degenerative="" drug="" extending="" fatty="" from="" further="" group="" groups="" gsh="" hepatocytes="" hepatoprotective="" hepatotoxicity.="" hepatoxicity.="" histopathology="" in="" increasing="" indicate="" induced="" infiltration="" is="" its="" kg="" level="" levels="" liver="" mda="" mg="" microvesicular="" mononuclear="" near="" necrosis="" needed="" normal="" nse="" of="" one="" p="" persisting="" portal="" possesses="" pre-treatment="" prevention="" regaining="" research="" results="" rif="" serum="" showed="" sod="" span="" steatosis.="" sub="" that="" the="" to="" tract.="" tract="" triditis="" use="" with="">

Black cumin seeds possess analgesic and anti-inflammatory activity comparable to indomethacin.

IN VIVO EVALUATION OF ANTI-INFLAMMATORY AND ANALGESIC ACTIVITIES OF NIGELLA SATIVA SEED DURING GERMINATION

MOHAMMAD HAYATUL ISLAM, IFFAT ZAREEN AHMAD AND MOHAMMAD TARIQ SALMAN

Objective: The medicinal values of Nigella sativa have been mentioned in ancient literature as useful in disorders of inflammation. The present study investigates the anti-inflammatory and analgesic activities of methanol extract of Nigella sativa seed during different phases of its germination on Wistar rats. Methods: Seeds of N. sativa were grown in vitro in glass petri plates using multiple folds of damp filter paper. Complete plantlet with two leaves was obtained in 11 days. The acute anti-inflammatory activity of N. sativa extracts during different phases of germination was measured plethysmographically using kaolin as inflammatory agent, analgesic activity was measured by hot plate method, keeping indomethacin (10mg/kg b.w) as reference standard in both tests.
Results: All tested extracts of N. sativa (1gm/kg b.w) during different phases of germination showed significant reduction in paw oedema in comparison to control (P<0 .001="" 5th="" all="" among="" analgesic="" and="" anti-inflammatory="" day="" during="" effect="" extract="" extracts.="" germination="" hot="" in="" increased="" of="" on="" p="" plate="" reaction="" showed="" significant="" test.="" test="" tested="" the="" time="">
Conclusion: It may be concluded that extracts of N. sativa possess enhanced anti-inflammatory and analgesic activities during germination as compared to seed extract. High metabolic activity and higher contents of secondary metabolites expressed during germination might be responsible for this activity.

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Published article: The heart and cardiovascular system in the Qur'an and Hadeeth

This published article entitled "The heart and cardiovascular system in the Qur'an and Hadeeth" taken from "International Journal of Cardiology" having impact factor of 3.1
(http://www.elsevier.com/wps/find/journaldescription.cws_home/506041/description#)

In this paper it has been clearly mentioned:

"The Islamic empire, for more than 1000 years, remained the most advanced and civilized empire in the world, and the inspiration of all the scientific and medical discoveries and practices stemmed from the teachings of the Qur'an and the Hadeeth, teachings that strongly encouraged and supported the drive to seek knowledge and to make scientific achievements and discoveries."

A Study of Nigella sativa Linn. seeds for antimicrobial activity against multidrug resistant clinical strains of Pseudomonas aeruginosa.

A Study of Nigella sativa Linn. seeds for antimicrobial activity against multidrug resistant clinical strains of Pseudomonas aeruginosa. Hippocratic Journal of Unani Medicine. 4(4). 2009. 95-104.
Authors: Mohd T Salman(1),*, Rahat A Khan(1), Indu Shukla(2)
Address: (1)Department of Pharmacology, (2)Department of Microbiology, Jawaharlal Nehru Medical College, Aligarh Muslim University, Aligarh, India. 202002.
*Corresponding Author: Present address- Department of Pharmacology, Era’s Lucknow Medical College, Sarfarazganj, Hardoi Road, Lucknow. India. 202003.
Abstract
Nigella sativa (black cumin) seed oil and extracts were tested in varying dilutions against strains of Pseudomonas aeruginosa resistant to a number of clinically used antibiotics isolated from patients attending JN Medical College Hospital, Aligarh, using disc agar diffusion technique on inoculated Muellar Hinton agar plates under standard laboratory conditions. Both the oil and Methanolic extract showed remarkable dose dependant antibacterial activity against the tested strains upto a dilution of 1:50 as evident from the zones of inhibition. No cross resistance was noticed with any of the tested antibiotics.

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